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Cell-matrix interaction which is critically important in physiological conditions such as growth, development wound repair and is altered under a number of pathological conditions. Employing Biochemical, immunological cytochemical, cell culture and molecular biology techniques in different model systems, he has been able to demonstrate that the extra cellular matrix, apart from providing structural support for tissue architecture, tremendously influences cellular function. He has contributed significantly to the field of matrix by studying the production of glycoprotein’s and proteoglycans of the extra cellular matrix by cells and demonstrating the modulation of the biochemical activity of liver cells , differentiated function of cells of mammary gland and the scavenging activity of macrophages by extra cellular matrix . He further studied the molecular basis of cell matrix interaction by identifying the cell surface receptors of matrix proteins and certain events in the downstream intracellular signaling process. He could also identify neutral proteases that degrade matrix proteins and the mechanisms of their regulation which are particularly relevant in the control of the microenvironment of the cell, cell social behavior and tissue remodelling such as during liver regeneration and mammary gland involution. Apart from their physiological significance, these findings are important in the pathobiochemistry of fibrotic diseases such as hepatic fibrosis, atherosclerosis, tumour metastasis and arthritis. Using reconstituted three dimensional biomatrix support, he could grow liver cells in vitro, which has tremendous application in tissue engineering
Cell biology of lipoprotein production by liver cells which is a complex process involving synthesis of different lipids, proteins, their assembly and secretion. Apart from demonstrating a critical role for nascently formed cholesterol in the assembly and the secretion of very low density lipoproteins by liver cells, I also shown that prostaglandins regulate lipo protein production. This provided a molecular basis for the beneficial lipid lowering effect of fish consumption. In a major production based cross sectional study which confirmed the beneficial effect of fish consumption, he has demonstrated correlation between diet and life style and oxidant and antioxidant status of plasma and buccal cells which are particularly significant in the risk assessment for chronic diseases such as CVD and oral cancer and suggested food and nutrient based intervention strategies
In another major study is relating to Vitamin A deficiency , a major public health problem , My group contributed significantly by developing technologies for studying the bioavailability of provitamin A by producing deuterated β-carotene by metallic labeling and by using non invasive techniques where exfoliated colonic epithelial cells can be used to assess bioconversion of provitamin A. Other contribution which deserves particular mention is the discovery of certain naturally occurring substances which have remarkable liver protective effect, antithrombotic and antiatherosclerotic effect
Research Activities :
Extracellular Matrix in Liver
Cell Surface herparan sulphate proteoglycan
Modulation of Biochemical activity of cells by ECM
Blood Cell-vessel wall Interaction
Matrix metalloproteinases in mammary gland remodelling
Regulation of VLDL Metabolism
Cylanoglycoside metabolism in cassava
Bioavailability of Vitamin A